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biotin anti human cd25 goat polyclonal antibody  (R&D Systems)


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    R&D Systems biotin anti human cd25 goat polyclonal antibody
    Biotin Anti Human Cd25 Goat Polyclonal Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotin+anti+human+cd25+goat+polyclonal+antibody/Human+CD25%2FIL-2R+alpha+Biotinylated+Antibody/pm41308987-150-31-36
    Average 94 stars, based on 12 article reviews
    biotin anti human cd25 goat polyclonal antibody - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Mutagenesis:

    Article Title: A CD25-chemokine receptor complex initiates non-canonical IL-2 signaling.
    Article Snippet: Anti-CCR7 polyclonal antibody (Novus Biologicals #NBP2-67324), anti-HA mouse monoclonal antibody (12CA5), anti-HA mouse monoclonal antibody (BioLegend #900801), anti-HA rabbit polyclonal antibody (BioLegend #923501), biotin-anti-human CCR7 mouse monoclonal antibody (BioLegend 210 #353240), biotin-anti-human CD25 goat polyclonal antibody (R&D Systems # BAF223), biotin-anti-mouse CD25 goat polyclonal antibody (R&D Systems # BAF2438), anti-mouse CD25 monoclonal antibody (clone PC-61.5.3, BioXcell # BE0012), anti-human CD25 monoclonal antibody (7G7B6)(17), anti-human CD25 monoclonal antibody (BC96, BioLegend #302609), Streptavidin-horseradish peroxidase (Invitrogen, #S911), IRDye® 800CW Goat anti-Mouse IgG Secondary Antibody (LI-COR 926-32210), IRDye® 800CW Goat anti-215 Rabbit IgG Secondary Antibody (LI-COR #926-32211), IRDye® 680RD Goat anti-Rabbit IgG Secondary Antibody (LI-COR #926-68071), IRDye® 680RD Goat anti-Mouse IgG Secondary Antibody (LI-COR #926- 68070), Protein G agarose (GenScript # L00209).

    Article Title: A CD25-CCR7 complex initiates non-canonical IL-2 signaling
    Article Snippet: Anti-CCR7 polyclonal antibody (Novus Biologicals #NBP2-67324), anti-HA mouse monoclonal antibody (12CA5), anti-HA mouse monoclonal antibody (BioLegend #900801), anti-HA rabbit polyclonal antibody (BioLegend #923501), biotin-anti-human CCR7 mouse monoclonal antibody (BioLegend #353240), biotin-anti-human CD25 goat polyclonal antibody (R&D Systems # BAF223), biotin-anti-mouse CD25 goat polyclonal antibody (R&D Systems # BAF2438), anti-mouse CD25 monoclonal antibody (clone PC-61.5.3, BioXcell # BE0012), anti-human CD25 monoclonal antibody (7G7B6) , anti-human CD25 monoclonal antibody (BC96, BioLegend #302609), Streptavidin-horseradish peroxidase (Invitrogen, #S911), IRDye® 800CW Goat anti-Mouse IgG Secondary Antibody (LI-COR 926-32210), IRDye® 800CW Goat anti-Rabbit IgG Secondary Antibody (LI-COR #926-32211), IRDye® 680RD Goat anti-Rabbit IgG Secondary Antibody (LI-COR #926-68071), IRDye® 680RD Goat anti-Mouse IgG Secondary Antibody (LI-COR #926-68070), Protein G agarose (GenScript # L00209).

    Enzyme-linked Immunosorbent Assay:

    Article Title: A CD25-chemokine receptor complex initiates non-canonical IL-2 signaling.
    Article Snippet: Anti-CCR7 polyclonal antibody (Novus Biologicals #NBP2-67324), anti-HA mouse monoclonal antibody (12CA5), anti-HA mouse monoclonal antibody (BioLegend #900801), anti-HA rabbit polyclonal antibody (BioLegend #923501), biotin-anti-human CCR7 mouse monoclonal antibody (BioLegend 210 #353240), biotin-anti-human CD25 goat polyclonal antibody (R&D Systems # BAF223), biotin-anti-mouse CD25 goat polyclonal antibody (R&D Systems # BAF2438), anti-mouse CD25 monoclonal antibody (clone PC-61.5.3, BioXcell # BE0012), anti-human CD25 monoclonal antibody (7G7B6)(17), anti-human CD25 monoclonal antibody (BC96, BioLegend #302609), Streptavidin-horseradish peroxidase (Invitrogen, #S911), IRDye® 800CW Goat anti-Mouse IgG Secondary Antibody (LI-COR 926-32210), IRDye® 800CW Goat anti-215 Rabbit IgG Secondary Antibody (LI-COR #926-32211), IRDye® 680RD Goat anti-Rabbit IgG Secondary Antibody (LI-COR #926-68071), IRDye® 680RD Goat anti-Mouse IgG Secondary Antibody (LI-COR #926- 68070), Protein G agarose (GenScript # L00209).

    Article Title: A CD25-CCR7 complex initiates non-canonical IL-2 signaling
    Article Snippet: Anti-CCR7 polyclonal antibody (Novus Biologicals #NBP2-67324), anti-HA mouse monoclonal antibody (12CA5), anti-HA mouse monoclonal antibody (BioLegend #900801), anti-HA rabbit polyclonal antibody (BioLegend #923501), biotin-anti-human CCR7 mouse monoclonal antibody (BioLegend #353240), biotin-anti-human CD25 goat polyclonal antibody (R&D Systems # BAF223), biotin-anti-mouse CD25 goat polyclonal antibody (R&D Systems # BAF2438), anti-mouse CD25 monoclonal antibody (clone PC-61.5.3, BioXcell # BE0012), anti-human CD25 monoclonal antibody (7G7B6) , anti-human CD25 monoclonal antibody (BC96, BioLegend #302609), Streptavidin-horseradish peroxidase (Invitrogen, #S911), IRDye® 800CW Goat anti-Mouse IgG Secondary Antibody (LI-COR 926-32210), IRDye® 800CW Goat anti-Rabbit IgG Secondary Antibody (LI-COR #926-32211), IRDye® 680RD Goat anti-Rabbit IgG Secondary Antibody (LI-COR #926-68071), IRDye® 680RD Goat anti-Mouse IgG Secondary Antibody (LI-COR #926-68070), Protein G agarose (GenScript # L00209).

    Incubation:

    Article Title: A CD25-chemokine receptor complex initiates non-canonical IL-2 signaling.
    Article Snippet: Anti-CCR7 polyclonal antibody (Novus Biologicals #NBP2-67324), anti-HA mouse monoclonal antibody (12CA5), anti-HA mouse monoclonal antibody (BioLegend #900801), anti-HA rabbit polyclonal antibody (BioLegend #923501), biotin-anti-human CCR7 mouse monoclonal antibody (BioLegend 210 #353240), biotin-anti-human CD25 goat polyclonal antibody (R&D Systems # BAF223), biotin-anti-mouse CD25 goat polyclonal antibody (R&D Systems # BAF2438), anti-mouse CD25 monoclonal antibody (clone PC-61.5.3, BioXcell # BE0012), anti-human CD25 monoclonal antibody (7G7B6)(17), anti-human CD25 monoclonal antibody (BC96, BioLegend #302609), Streptavidin-horseradish peroxidase (Invitrogen, #S911), IRDye® 800CW Goat anti-Mouse IgG Secondary Antibody (LI-COR 926-32210), IRDye® 800CW Goat anti-215 Rabbit IgG Secondary Antibody (LI-COR #926-32211), IRDye® 680RD Goat anti-Rabbit IgG Secondary Antibody (LI-COR #926-68071), IRDye® 680RD Goat anti-Mouse IgG Secondary Antibody (LI-COR #926- 68070), Protein G agarose (GenScript # L00209).

    Article Title: A CD25-CCR7 complex initiates non-canonical IL-2 signaling
    Article Snippet: Anti-CCR7 polyclonal antibody (Novus Biologicals #NBP2-67324), anti-HA mouse monoclonal antibody (12CA5), anti-HA mouse monoclonal antibody (BioLegend #900801), anti-HA rabbit polyclonal antibody (BioLegend #923501), biotin-anti-human CCR7 mouse monoclonal antibody (BioLegend #353240), biotin-anti-human CD25 goat polyclonal antibody (R&D Systems # BAF223), biotin-anti-mouse CD25 goat polyclonal antibody (R&D Systems # BAF2438), anti-mouse CD25 monoclonal antibody (clone PC-61.5.3, BioXcell # BE0012), anti-human CD25 monoclonal antibody (7G7B6) , anti-human CD25 monoclonal antibody (BC96, BioLegend #302609), Streptavidin-horseradish peroxidase (Invitrogen, #S911), IRDye® 800CW Goat anti-Mouse IgG Secondary Antibody (LI-COR 926-32210), IRDye® 800CW Goat anti-Rabbit IgG Secondary Antibody (LI-COR #926-32211), IRDye® 680RD Goat anti-Rabbit IgG Secondary Antibody (LI-COR #926-68071), IRDye® 680RD Goat anti-Mouse IgG Secondary Antibody (LI-COR #926-68070), Protein G agarose (GenScript # L00209).

    Immunoprecipitation:

    Article Title: A CD25-chemokine receptor complex initiates non-canonical IL-2 signaling.
    Article Snippet: Anti-CCR7 polyclonal antibody (Novus Biologicals #NBP2-67324), anti-HA mouse monoclonal antibody (12CA5), anti-HA mouse monoclonal antibody (BioLegend #900801), anti-HA rabbit polyclonal antibody (BioLegend #923501), biotin-anti-human CCR7 mouse monoclonal antibody (BioLegend 210 #353240), biotin-anti-human CD25 goat polyclonal antibody (R&D Systems # BAF223), biotin-anti-mouse CD25 goat polyclonal antibody (R&D Systems # BAF2438), anti-mouse CD25 monoclonal antibody (clone PC-61.5.3, BioXcell # BE0012), anti-human CD25 monoclonal antibody (7G7B6)(17), anti-human CD25 monoclonal antibody (BC96, BioLegend #302609), Streptavidin-horseradish peroxidase (Invitrogen, #S911), IRDye® 800CW Goat anti-Mouse IgG Secondary Antibody (LI-COR 926-32210), IRDye® 800CW Goat anti-215 Rabbit IgG Secondary Antibody (LI-COR #926-32211), IRDye® 680RD Goat anti-Rabbit IgG Secondary Antibody (LI-COR #926-68071), IRDye® 680RD Goat anti-Mouse IgG Secondary Antibody (LI-COR #926- 68070), Protein G agarose (GenScript # L00209).

    Article Title: A CD25-CCR7 complex initiates non-canonical IL-2 signaling
    Article Snippet: Anti-CCR7 polyclonal antibody (Novus Biologicals #NBP2-67324), anti-HA mouse monoclonal antibody (12CA5), anti-HA mouse monoclonal antibody (BioLegend #900801), anti-HA rabbit polyclonal antibody (BioLegend #923501), biotin-anti-human CCR7 mouse monoclonal antibody (BioLegend #353240), biotin-anti-human CD25 goat polyclonal antibody (R&D Systems # BAF223), biotin-anti-mouse CD25 goat polyclonal antibody (R&D Systems # BAF2438), anti-mouse CD25 monoclonal antibody (clone PC-61.5.3, BioXcell # BE0012), anti-human CD25 monoclonal antibody (7G7B6) , anti-human CD25 monoclonal antibody (BC96, BioLegend #302609), Streptavidin-horseradish peroxidase (Invitrogen, #S911), IRDye® 800CW Goat anti-Mouse IgG Secondary Antibody (LI-COR 926-32210), IRDye® 800CW Goat anti-Rabbit IgG Secondary Antibody (LI-COR #926-32211), IRDye® 680RD Goat anti-Rabbit IgG Secondary Antibody (LI-COR #926-68071), IRDye® 680RD Goat anti-Mouse IgG Secondary Antibody (LI-COR #926-68070), Protein G agarose (GenScript # L00209).

    Western Blot:

    Article Title: A CD25-chemokine receptor complex initiates non-canonical IL-2 signaling.
    Article Snippet: Anti-CCR7 polyclonal antibody (Novus Biologicals #NBP2-67324), anti-HA mouse monoclonal antibody (12CA5), anti-HA mouse monoclonal antibody (BioLegend #900801), anti-HA rabbit polyclonal antibody (BioLegend #923501), biotin-anti-human CCR7 mouse monoclonal antibody (BioLegend 210 #353240), biotin-anti-human CD25 goat polyclonal antibody (R&D Systems # BAF223), biotin-anti-mouse CD25 goat polyclonal antibody (R&D Systems # BAF2438), anti-mouse CD25 monoclonal antibody (clone PC-61.5.3, BioXcell # BE0012), anti-human CD25 monoclonal antibody (7G7B6)(17), anti-human CD25 monoclonal antibody (BC96, BioLegend #302609), Streptavidin-horseradish peroxidase (Invitrogen, #S911), IRDye® 800CW Goat anti-Mouse IgG Secondary Antibody (LI-COR 926-32210), IRDye® 800CW Goat anti-215 Rabbit IgG Secondary Antibody (LI-COR #926-32211), IRDye® 680RD Goat anti-Rabbit IgG Secondary Antibody (LI-COR #926-68071), IRDye® 680RD Goat anti-Mouse IgG Secondary Antibody (LI-COR #926- 68070), Protein G agarose (GenScript # L00209).

    Article Title: A CD25-CCR7 complex initiates non-canonical IL-2 signaling
    Article Snippet: Anti-CCR7 polyclonal antibody (Novus Biologicals #NBP2-67324), anti-HA mouse monoclonal antibody (12CA5), anti-HA mouse monoclonal antibody (BioLegend #900801), anti-HA rabbit polyclonal antibody (BioLegend #923501), biotin-anti-human CCR7 mouse monoclonal antibody (BioLegend #353240), biotin-anti-human CD25 goat polyclonal antibody (R&D Systems # BAF223), biotin-anti-mouse CD25 goat polyclonal antibody (R&D Systems # BAF2438), anti-mouse CD25 monoclonal antibody (clone PC-61.5.3, BioXcell # BE0012), anti-human CD25 monoclonal antibody (7G7B6) , anti-human CD25 monoclonal antibody (BC96, BioLegend #302609), Streptavidin-horseradish peroxidase (Invitrogen, #S911), IRDye® 800CW Goat anti-Mouse IgG Secondary Antibody (LI-COR 926-32210), IRDye® 800CW Goat anti-Rabbit IgG Secondary Antibody (LI-COR #926-32211), IRDye® 680RD Goat anti-Rabbit IgG Secondary Antibody (LI-COR #926-68071), IRDye® 680RD Goat anti-Mouse IgG Secondary Antibody (LI-COR #926-68070), Protein G agarose (GenScript # L00209).

    Expressing:

    Article Title: A CD25-chemokine receptor complex initiates non-canonical IL-2 signaling.
    Article Snippet: Anti-CCR7 polyclonal antibody (Novus Biologicals #NBP2-67324), anti-HA mouse monoclonal antibody (12CA5), anti-HA mouse monoclonal antibody (BioLegend #900801), anti-HA rabbit polyclonal antibody (BioLegend #923501), biotin-anti-human CCR7 mouse monoclonal antibody (BioLegend 210 #353240), biotin-anti-human CD25 goat polyclonal antibody (R&D Systems # BAF223), biotin-anti-mouse CD25 goat polyclonal antibody (R&D Systems # BAF2438), anti-mouse CD25 monoclonal antibody (clone PC-61.5.3, BioXcell # BE0012), anti-human CD25 monoclonal antibody (7G7B6)(17), anti-human CD25 monoclonal antibody (BC96, BioLegend #302609), Streptavidin-horseradish peroxidase (Invitrogen, #S911), IRDye® 800CW Goat anti-Mouse IgG Secondary Antibody (LI-COR 926-32210), IRDye® 800CW Goat anti-215 Rabbit IgG Secondary Antibody (LI-COR #926-32211), IRDye® 680RD Goat anti-Rabbit IgG Secondary Antibody (LI-COR #926-68071), IRDye® 680RD Goat anti-Mouse IgG Secondary Antibody (LI-COR #926- 68070), Protein G agarose (GenScript # L00209).

    Article Title: A CD25-CCR7 complex initiates non-canonical IL-2 signaling
    Article Snippet: Anti-CCR7 polyclonal antibody (Novus Biologicals #NBP2-67324), anti-HA mouse monoclonal antibody (12CA5), anti-HA mouse monoclonal antibody (BioLegend #900801), anti-HA rabbit polyclonal antibody (BioLegend #923501), biotin-anti-human CCR7 mouse monoclonal antibody (BioLegend #353240), biotin-anti-human CD25 goat polyclonal antibody (R&D Systems # BAF223), biotin-anti-mouse CD25 goat polyclonal antibody (R&D Systems # BAF2438), anti-mouse CD25 monoclonal antibody (clone PC-61.5.3, BioXcell # BE0012), anti-human CD25 monoclonal antibody (7G7B6) , anti-human CD25 monoclonal antibody (BC96, BioLegend #302609), Streptavidin-horseradish peroxidase (Invitrogen, #S911), IRDye® 800CW Goat anti-Mouse IgG Secondary Antibody (LI-COR 926-32210), IRDye® 800CW Goat anti-Rabbit IgG Secondary Antibody (LI-COR #926-32211), IRDye® 680RD Goat anti-Rabbit IgG Secondary Antibody (LI-COR #926-68071), IRDye® 680RD Goat anti-Mouse IgG Secondary Antibody (LI-COR #926-68070), Protein G agarose (GenScript # L00209).

    Lysis:

    Article Title: A CD25-chemokine receptor complex initiates non-canonical IL-2 signaling.
    Article Snippet: Anti-CCR7 polyclonal antibody (Novus Biologicals #NBP2-67324), anti-HA mouse monoclonal antibody (12CA5), anti-HA mouse monoclonal antibody (BioLegend #900801), anti-HA rabbit polyclonal antibody (BioLegend #923501), biotin-anti-human CCR7 mouse monoclonal antibody (BioLegend 210 #353240), biotin-anti-human CD25 goat polyclonal antibody (R&D Systems # BAF223), biotin-anti-mouse CD25 goat polyclonal antibody (R&D Systems # BAF2438), anti-mouse CD25 monoclonal antibody (clone PC-61.5.3, BioXcell # BE0012), anti-human CD25 monoclonal antibody (7G7B6)(17), anti-human CD25 monoclonal antibody (BC96, BioLegend #302609), Streptavidin-horseradish peroxidase (Invitrogen, #S911), IRDye® 800CW Goat anti-Mouse IgG Secondary Antibody (LI-COR 926-32210), IRDye® 800CW Goat anti-215 Rabbit IgG Secondary Antibody (LI-COR #926-32211), IRDye® 680RD Goat anti-Rabbit IgG Secondary Antibody (LI-COR #926-68071), IRDye® 680RD Goat anti-Mouse IgG Secondary Antibody (LI-COR #926- 68070), Protein G agarose (GenScript # L00209).

    Article Title: A CD25-CCR7 complex initiates non-canonical IL-2 signaling
    Article Snippet: Anti-CCR7 polyclonal antibody (Novus Biologicals #NBP2-67324), anti-HA mouse monoclonal antibody (12CA5), anti-HA mouse monoclonal antibody (BioLegend #900801), anti-HA rabbit polyclonal antibody (BioLegend #923501), biotin-anti-human CCR7 mouse monoclonal antibody (BioLegend #353240), biotin-anti-human CD25 goat polyclonal antibody (R&D Systems # BAF223), biotin-anti-mouse CD25 goat polyclonal antibody (R&D Systems # BAF2438), anti-mouse CD25 monoclonal antibody (clone PC-61.5.3, BioXcell # BE0012), anti-human CD25 monoclonal antibody (7G7B6) , anti-human CD25 monoclonal antibody (BC96, BioLegend #302609), Streptavidin-horseradish peroxidase (Invitrogen, #S911), IRDye® 800CW Goat anti-Mouse IgG Secondary Antibody (LI-COR 926-32210), IRDye® 800CW Goat anti-Rabbit IgG Secondary Antibody (LI-COR #926-32211), IRDye® 680RD Goat anti-Rabbit IgG Secondary Antibody (LI-COR #926-68071), IRDye® 680RD Goat anti-Mouse IgG Secondary Antibody (LI-COR #926-68070), Protein G agarose (GenScript # L00209).

    Recombinant:

    Article Title: A CD25-chemokine receptor complex initiates non-canonical IL-2 signaling.
    Article Snippet: Anti-CCR7 polyclonal antibody (Novus Biologicals #NBP2-67324), anti-HA mouse monoclonal antibody (12CA5), anti-HA mouse monoclonal antibody (BioLegend #900801), anti-HA rabbit polyclonal antibody (BioLegend #923501), biotin-anti-human CCR7 mouse monoclonal antibody (BioLegend 210 #353240), biotin-anti-human CD25 goat polyclonal antibody (R&D Systems # BAF223), biotin-anti-mouse CD25 goat polyclonal antibody (R&D Systems # BAF2438), anti-mouse CD25 monoclonal antibody (clone PC-61.5.3, BioXcell # BE0012), anti-human CD25 monoclonal antibody (7G7B6)(17), anti-human CD25 monoclonal antibody (BC96, BioLegend #302609), Streptavidin-horseradish peroxidase (Invitrogen, #S911), IRDye® 800CW Goat anti-Mouse IgG Secondary Antibody (LI-COR 926-32210), IRDye® 800CW Goat anti-215 Rabbit IgG Secondary Antibody (LI-COR #926-32211), IRDye® 680RD Goat anti-Rabbit IgG Secondary Antibody (LI-COR #926-68071), IRDye® 680RD Goat anti-Mouse IgG Secondary Antibody (LI-COR #926- 68070), Protein G agarose (GenScript # L00209).

    Article Title: A CD25-CCR7 complex initiates non-canonical IL-2 signaling
    Article Snippet: Anti-CCR7 polyclonal antibody (Novus Biologicals #NBP2-67324), anti-HA mouse monoclonal antibody (12CA5), anti-HA mouse monoclonal antibody (BioLegend #900801), anti-HA rabbit polyclonal antibody (BioLegend #923501), biotin-anti-human CCR7 mouse monoclonal antibody (BioLegend #353240), biotin-anti-human CD25 goat polyclonal antibody (R&D Systems # BAF223), biotin-anti-mouse CD25 goat polyclonal antibody (R&D Systems # BAF2438), anti-mouse CD25 monoclonal antibody (clone PC-61.5.3, BioXcell # BE0012), anti-human CD25 monoclonal antibody (7G7B6) , anti-human CD25 monoclonal antibody (BC96, BioLegend #302609), Streptavidin-horseradish peroxidase (Invitrogen, #S911), IRDye® 800CW Goat anti-Mouse IgG Secondary Antibody (LI-COR 926-32210), IRDye® 800CW Goat anti-Rabbit IgG Secondary Antibody (LI-COR #926-32211), IRDye® 680RD Goat anti-Rabbit IgG Secondary Antibody (LI-COR #926-68071), IRDye® 680RD Goat anti-Mouse IgG Secondary Antibody (LI-COR #926-68070), Protein G agarose (GenScript # L00209).

    Activation Assay:

    Article Title: A CD25-chemokine receptor complex initiates non-canonical IL-2 signaling.
    Article Snippet: Anti-CCR7 polyclonal antibody (Novus Biologicals #NBP2-67324), anti-HA mouse monoclonal antibody (12CA5), anti-HA mouse monoclonal antibody (BioLegend #900801), anti-HA rabbit polyclonal antibody (BioLegend #923501), biotin-anti-human CCR7 mouse monoclonal antibody (BioLegend 210 #353240), biotin-anti-human CD25 goat polyclonal antibody (R&D Systems # BAF223), biotin-anti-mouse CD25 goat polyclonal antibody (R&D Systems # BAF2438), anti-mouse CD25 monoclonal antibody (clone PC-61.5.3, BioXcell # BE0012), anti-human CD25 monoclonal antibody (7G7B6)(17), anti-human CD25 monoclonal antibody (BC96, BioLegend #302609), Streptavidin-horseradish peroxidase (Invitrogen, #S911), IRDye® 800CW Goat anti-Mouse IgG Secondary Antibody (LI-COR 926-32210), IRDye® 800CW Goat anti-215 Rabbit IgG Secondary Antibody (LI-COR #926-32211), IRDye® 680RD Goat anti-Rabbit IgG Secondary Antibody (LI-COR #926-68071), IRDye® 680RD Goat anti-Mouse IgG Secondary Antibody (LI-COR #926- 68070), Protein G agarose (GenScript # L00209).

    Article Title: A CD25-CCR7 complex initiates non-canonical IL-2 signaling
    Article Snippet: Anti-CCR7 polyclonal antibody (Novus Biologicals #NBP2-67324), anti-HA mouse monoclonal antibody (12CA5), anti-HA mouse monoclonal antibody (BioLegend #900801), anti-HA rabbit polyclonal antibody (BioLegend #923501), biotin-anti-human CCR7 mouse monoclonal antibody (BioLegend #353240), biotin-anti-human CD25 goat polyclonal antibody (R&D Systems # BAF223), biotin-anti-mouse CD25 goat polyclonal antibody (R&D Systems # BAF2438), anti-mouse CD25 monoclonal antibody (clone PC-61.5.3, BioXcell # BE0012), anti-human CD25 monoclonal antibody (7G7B6) , anti-human CD25 monoclonal antibody (BC96, BioLegend #302609), Streptavidin-horseradish peroxidase (Invitrogen, #S911), IRDye® 800CW Goat anti-Mouse IgG Secondary Antibody (LI-COR 926-32210), IRDye® 800CW Goat anti-Rabbit IgG Secondary Antibody (LI-COR #926-32211), IRDye® 680RD Goat anti-Rabbit IgG Secondary Antibody (LI-COR #926-68071), IRDye® 680RD Goat anti-Mouse IgG Secondary Antibody (LI-COR #926-68070), Protein G agarose (GenScript # L00209).

    Binding Assay:

    Article Title: A CD25-chemokine receptor complex initiates non-canonical IL-2 signaling.
    Article Snippet: Anti-CCR7 polyclonal antibody (Novus Biologicals #NBP2-67324), anti-HA mouse monoclonal antibody (12CA5), anti-HA mouse monoclonal antibody (BioLegend #900801), anti-HA rabbit polyclonal antibody (BioLegend #923501), biotin-anti-human CCR7 mouse monoclonal antibody (BioLegend 210 #353240), biotin-anti-human CD25 goat polyclonal antibody (R&D Systems # BAF223), biotin-anti-mouse CD25 goat polyclonal antibody (R&D Systems # BAF2438), anti-mouse CD25 monoclonal antibody (clone PC-61.5.3, BioXcell # BE0012), anti-human CD25 monoclonal antibody (7G7B6)(17), anti-human CD25 monoclonal antibody (BC96, BioLegend #302609), Streptavidin-horseradish peroxidase (Invitrogen, #S911), IRDye® 800CW Goat anti-Mouse IgG Secondary Antibody (LI-COR 926-32210), IRDye® 800CW Goat anti-215 Rabbit IgG Secondary Antibody (LI-COR #926-32211), IRDye® 680RD Goat anti-Rabbit IgG Secondary Antibody (LI-COR #926-68071), IRDye® 680RD Goat anti-Mouse IgG Secondary Antibody (LI-COR #926- 68070), Protein G agarose (GenScript # L00209).

    Article Title: A CD25-CCR7 complex initiates non-canonical IL-2 signaling
    Article Snippet: Anti-CCR7 polyclonal antibody (Novus Biologicals #NBP2-67324), anti-HA mouse monoclonal antibody (12CA5), anti-HA mouse monoclonal antibody (BioLegend #900801), anti-HA rabbit polyclonal antibody (BioLegend #923501), biotin-anti-human CCR7 mouse monoclonal antibody (BioLegend #353240), biotin-anti-human CD25 goat polyclonal antibody (R&D Systems # BAF223), biotin-anti-mouse CD25 goat polyclonal antibody (R&D Systems # BAF2438), anti-mouse CD25 monoclonal antibody (clone PC-61.5.3, BioXcell # BE0012), anti-human CD25 monoclonal antibody (7G7B6) , anti-human CD25 monoclonal antibody (BC96, BioLegend #302609), Streptavidin-horseradish peroxidase (Invitrogen, #S911), IRDye® 800CW Goat anti-Mouse IgG Secondary Antibody (LI-COR 926-32210), IRDye® 800CW Goat anti-Rabbit IgG Secondary Antibody (LI-COR #926-32211), IRDye® 680RD Goat anti-Rabbit IgG Secondary Antibody (LI-COR #926-68071), IRDye® 680RD Goat anti-Mouse IgG Secondary Antibody (LI-COR #926-68070), Protein G agarose (GenScript # L00209).

    Isolation:

    Article Title: A CD25-chemokine receptor complex initiates non-canonical IL-2 signaling.
    Article Snippet: Anti-CCR7 polyclonal antibody (Novus Biologicals #NBP2-67324), anti-HA mouse monoclonal antibody (12CA5), anti-HA mouse monoclonal antibody (BioLegend #900801), anti-HA rabbit polyclonal antibody (BioLegend #923501), biotin-anti-human CCR7 mouse monoclonal antibody (BioLegend 210 #353240), biotin-anti-human CD25 goat polyclonal antibody (R&D Systems # BAF223), biotin-anti-mouse CD25 goat polyclonal antibody (R&D Systems # BAF2438), anti-mouse CD25 monoclonal antibody (clone PC-61.5.3, BioXcell # BE0012), anti-human CD25 monoclonal antibody (7G7B6)(17), anti-human CD25 monoclonal antibody (BC96, BioLegend #302609), Streptavidin-horseradish peroxidase (Invitrogen, #S911), IRDye® 800CW Goat anti-Mouse IgG Secondary Antibody (LI-COR 926-32210), IRDye® 800CW Goat anti-215 Rabbit IgG Secondary Antibody (LI-COR #926-32211), IRDye® 680RD Goat anti-Rabbit IgG Secondary Antibody (LI-COR #926-68071), IRDye® 680RD Goat anti-Mouse IgG Secondary Antibody (LI-COR #926- 68070), Protein G agarose (GenScript # L00209).

    Article Title: A CD25-CCR7 complex initiates non-canonical IL-2 signaling
    Article Snippet: Anti-CCR7 polyclonal antibody (Novus Biologicals #NBP2-67324), anti-HA mouse monoclonal antibody (12CA5), anti-HA mouse monoclonal antibody (BioLegend #900801), anti-HA rabbit polyclonal antibody (BioLegend #923501), biotin-anti-human CCR7 mouse monoclonal antibody (BioLegend #353240), biotin-anti-human CD25 goat polyclonal antibody (R&D Systems # BAF223), biotin-anti-mouse CD25 goat polyclonal antibody (R&D Systems # BAF2438), anti-mouse CD25 monoclonal antibody (clone PC-61.5.3, BioXcell # BE0012), anti-human CD25 monoclonal antibody (7G7B6) , anti-human CD25 monoclonal antibody (BC96, BioLegend #302609), Streptavidin-horseradish peroxidase (Invitrogen, #S911), IRDye® 800CW Goat anti-Mouse IgG Secondary Antibody (LI-COR 926-32210), IRDye® 800CW Goat anti-Rabbit IgG Secondary Antibody (LI-COR #926-32211), IRDye® 680RD Goat anti-Rabbit IgG Secondary Antibody (LI-COR #926-68071), IRDye® 680RD Goat anti-Mouse IgG Secondary Antibody (LI-COR #926-68070), Protein G agarose (GenScript # L00209).

    Staining:

    Article Title: A CD25-chemokine receptor complex initiates non-canonical IL-2 signaling.
    Article Snippet: Anti-CCR7 polyclonal antibody (Novus Biologicals #NBP2-67324), anti-HA mouse monoclonal antibody (12CA5), anti-HA mouse monoclonal antibody (BioLegend #900801), anti-HA rabbit polyclonal antibody (BioLegend #923501), biotin-anti-human CCR7 mouse monoclonal antibody (BioLegend 210 #353240), biotin-anti-human CD25 goat polyclonal antibody (R&D Systems # BAF223), biotin-anti-mouse CD25 goat polyclonal antibody (R&D Systems # BAF2438), anti-mouse CD25 monoclonal antibody (clone PC-61.5.3, BioXcell # BE0012), anti-human CD25 monoclonal antibody (7G7B6)(17), anti-human CD25 monoclonal antibody (BC96, BioLegend #302609), Streptavidin-horseradish peroxidase (Invitrogen, #S911), IRDye® 800CW Goat anti-Mouse IgG Secondary Antibody (LI-COR 926-32210), IRDye® 800CW Goat anti-215 Rabbit IgG Secondary Antibody (LI-COR #926-32211), IRDye® 680RD Goat anti-Rabbit IgG Secondary Antibody (LI-COR #926-68071), IRDye® 680RD Goat anti-Mouse IgG Secondary Antibody (LI-COR #926- 68070), Protein G agarose (GenScript # L00209).

    Article Title: A CD25-CCR7 complex initiates non-canonical IL-2 signaling
    Article Snippet: Anti-CCR7 polyclonal antibody (Novus Biologicals #NBP2-67324), anti-HA mouse monoclonal antibody (12CA5), anti-HA mouse monoclonal antibody (BioLegend #900801), anti-HA rabbit polyclonal antibody (BioLegend #923501), biotin-anti-human CCR7 mouse monoclonal antibody (BioLegend #353240), biotin-anti-human CD25 goat polyclonal antibody (R&D Systems # BAF223), biotin-anti-mouse CD25 goat polyclonal antibody (R&D Systems # BAF2438), anti-mouse CD25 monoclonal antibody (clone PC-61.5.3, BioXcell # BE0012), anti-human CD25 monoclonal antibody (7G7B6) , anti-human CD25 monoclonal antibody (BC96, BioLegend #302609), Streptavidin-horseradish peroxidase (Invitrogen, #S911), IRDye® 800CW Goat anti-Mouse IgG Secondary Antibody (LI-COR 926-32210), IRDye® 800CW Goat anti-Rabbit IgG Secondary Antibody (LI-COR #926-32211), IRDye® 680RD Goat anti-Rabbit IgG Secondary Antibody (LI-COR #926-68071), IRDye® 680RD Goat anti-Mouse IgG Secondary Antibody (LI-COR #926-68070), Protein G agarose (GenScript # L00209).

    SDS Page:

    Article Title: A CD25-chemokine receptor complex initiates non-canonical IL-2 signaling.
    Article Snippet: Anti-CCR7 polyclonal antibody (Novus Biologicals #NBP2-67324), anti-HA mouse monoclonal antibody (12CA5), anti-HA mouse monoclonal antibody (BioLegend #900801), anti-HA rabbit polyclonal antibody (BioLegend #923501), biotin-anti-human CCR7 mouse monoclonal antibody (BioLegend 210 #353240), biotin-anti-human CD25 goat polyclonal antibody (R&D Systems # BAF223), biotin-anti-mouse CD25 goat polyclonal antibody (R&D Systems # BAF2438), anti-mouse CD25 monoclonal antibody (clone PC-61.5.3, BioXcell # BE0012), anti-human CD25 monoclonal antibody (7G7B6)(17), anti-human CD25 monoclonal antibody (BC96, BioLegend #302609), Streptavidin-horseradish peroxidase (Invitrogen, #S911), IRDye® 800CW Goat anti-Mouse IgG Secondary Antibody (LI-COR 926-32210), IRDye® 800CW Goat anti-215 Rabbit IgG Secondary Antibody (LI-COR #926-32211), IRDye® 680RD Goat anti-Rabbit IgG Secondary Antibody (LI-COR #926-68071), IRDye® 680RD Goat anti-Mouse IgG Secondary Antibody (LI-COR #926- 68070), Protein G agarose (GenScript # L00209).

    Article Title: A CD25-CCR7 complex initiates non-canonical IL-2 signaling
    Article Snippet: Anti-CCR7 polyclonal antibody (Novus Biologicals #NBP2-67324), anti-HA mouse monoclonal antibody (12CA5), anti-HA mouse monoclonal antibody (BioLegend #900801), anti-HA rabbit polyclonal antibody (BioLegend #923501), biotin-anti-human CCR7 mouse monoclonal antibody (BioLegend #353240), biotin-anti-human CD25 goat polyclonal antibody (R&D Systems # BAF223), biotin-anti-mouse CD25 goat polyclonal antibody (R&D Systems # BAF2438), anti-mouse CD25 monoclonal antibody (clone PC-61.5.3, BioXcell # BE0012), anti-human CD25 monoclonal antibody (7G7B6) , anti-human CD25 monoclonal antibody (BC96, BioLegend #302609), Streptavidin-horseradish peroxidase (Invitrogen, #S911), IRDye® 800CW Goat anti-Mouse IgG Secondary Antibody (LI-COR 926-32210), IRDye® 800CW Goat anti-Rabbit IgG Secondary Antibody (LI-COR #926-32211), IRDye® 680RD Goat anti-Rabbit IgG Secondary Antibody (LI-COR #926-68071), IRDye® 680RD Goat anti-Mouse IgG Secondary Antibody (LI-COR #926-68070), Protein G agarose (GenScript # L00209).

    Spectroscopy:

    Article Title: A CD25-chemokine receptor complex initiates non-canonical IL-2 signaling.
    Article Snippet: Anti-CCR7 polyclonal antibody (Novus Biologicals #NBP2-67324), anti-HA mouse monoclonal antibody (12CA5), anti-HA mouse monoclonal antibody (BioLegend #900801), anti-HA rabbit polyclonal antibody (BioLegend #923501), biotin-anti-human CCR7 mouse monoclonal antibody (BioLegend 210 #353240), biotin-anti-human CD25 goat polyclonal antibody (R&D Systems # BAF223), biotin-anti-mouse CD25 goat polyclonal antibody (R&D Systems # BAF2438), anti-mouse CD25 monoclonal antibody (clone PC-61.5.3, BioXcell # BE0012), anti-human CD25 monoclonal antibody (7G7B6)(17), anti-human CD25 monoclonal antibody (BC96, BioLegend #302609), Streptavidin-horseradish peroxidase (Invitrogen, #S911), IRDye® 800CW Goat anti-Mouse IgG Secondary Antibody (LI-COR 926-32210), IRDye® 800CW Goat anti-215 Rabbit IgG Secondary Antibody (LI-COR #926-32211), IRDye® 680RD Goat anti-Rabbit IgG Secondary Antibody (LI-COR #926-68071), IRDye® 680RD Goat anti-Mouse IgG Secondary Antibody (LI-COR #926- 68070), Protein G agarose (GenScript # L00209).

    Article Title: A CD25-CCR7 complex initiates non-canonical IL-2 signaling
    Article Snippet: Anti-CCR7 polyclonal antibody (Novus Biologicals #NBP2-67324), anti-HA mouse monoclonal antibody (12CA5), anti-HA mouse monoclonal antibody (BioLegend #900801), anti-HA rabbit polyclonal antibody (BioLegend #923501), biotin-anti-human CCR7 mouse monoclonal antibody (BioLegend #353240), biotin-anti-human CD25 goat polyclonal antibody (R&D Systems # BAF223), biotin-anti-mouse CD25 goat polyclonal antibody (R&D Systems # BAF2438), anti-mouse CD25 monoclonal antibody (clone PC-61.5.3, BioXcell # BE0012), anti-human CD25 monoclonal antibody (7G7B6) , anti-human CD25 monoclonal antibody (BC96, BioLegend #302609), Streptavidin-horseradish peroxidase (Invitrogen, #S911), IRDye® 800CW Goat anti-Mouse IgG Secondary Antibody (LI-COR 926-32210), IRDye® 800CW Goat anti-Rabbit IgG Secondary Antibody (LI-COR #926-32211), IRDye® 680RD Goat anti-Rabbit IgG Secondary Antibody (LI-COR #926-68071), IRDye® 680RD Goat anti-Mouse IgG Secondary Antibody (LI-COR #926-68070), Protein G agarose (GenScript # L00209).



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